US 7,612,257 B2 · Flavonoid 3′,5′ hydroxylase gene sequences and uses therefor

Abstract

The present invention relates generally to a genetic sequence encoding a polypeptide having flavonoid 3′,5′-hydroxylase (F3′5′H) activity and to the use of the genetic sequence and/or its corresponding polypeptide thereof inter alia to manipulate color in flowers or parts thereof or in other plant tissue. More particularly, the F3′5′H has the ability to modulate dihydrokaempferol (DHK) metabolism as well as the metabolism of other substrates such as dihydroquercetin (DHQ), naringenin and eriodictyol. Even more particularly, the present invention provides a genetic sequence encoding a polypeptide having F3′5′H activity when expressed in rose or gerbera or botanically related plants. The instant invention further relates to antisense and sense molecules or RNAi-inducing molecules corresponding to all or part of the subject genetic sequence or a transcript thereof. The present invention further relates to promoters which operate efficiently in plants such as rose, gerbera or botanically related plants.

Claims7

Dependent claims

An isolated nucleic acid molecule comprising a sequence of nucleotides encoding a flavonoid 3′,5′ hydroxylase (F3′5′H), said nucleotide sequence selected from the group consisting of:

(i) a nucleotide sequence encoding the amino acid sequence as set forth in SEQ ID NO:
12;
(ii) a nucleotide sequence encoding an amino acid sequence having at least 95% identity to the amino acid sequence as set forth in SEQ ID NO:
12;
(iii) the nucleotide sequence as set forth in SEQ ID NO:
11;
and
(iv) a nucleotide sequence capable of hybridizing to the complementary sequence of the nucleotide sequence as set forth in SEQ ID NO:
11 under high stringency conditions which comprise hybridization at 42° C. in 50% v/v formamide, 1M NaCl, 1% w/v SDS, and 10% w/v dextran sulphate, and washings at 65° C. first in 2×SSC, 1% w/v SDS and subsequently in 0.2×SSC, 1% w/v SDS.

6 more elements

Dependencies are inferred from the claim text.

Flavonoid 3′,5′ hydroxylase gene sequences and uses therefor

Life of this patent

Flavonoid 3′,5′ hydroxylase gene sequences and uses therefor

Life of this patent

Abstract

Claims7