Isolated nucleic acid molecules encoding the Br2 P-glycoprotein of maize and methods of modifying growth in plants transformed therewith
AssigneesPioneer Hi-Bred International, Inc., The Curators of the University of Missouri
InventorsGurmukh S. Johal, Dilbag S. Multani, Steven P. Briggs
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Abstract
The invention relates to the genetic modification of plants, particularly to the expression of P-glycoprotein genes in transformed plants. Nucleotide sequences for the Br2 gene encoding a P-glycoprotein of maize and methods for their use are provided. The sequences find use in modifying the growth of plants.
Claims13
An isolated nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence having at least 95% nucleotide sequence identity to the nucleotide sequence set forth in SEQ ID NO:
- 1;
- (b) a nucleotide sequence having at least 95% nucleotide sequence identity to the nucleotide sequence set forth in SEQ ID NO:
- 2;
- (c) a nucleotide sequence encoding a variant of the amino acid sequence set forth in SEQ ID NO:
- 3, wherein said variant has at least 90% amino acid sequence identity to the amino acid sequence set forth in SEQ ID NO:
- 3;
- and
- (d) a nucleotide sequence that is fully complementary to the nucleotide sequence of any one of (a)-(c);
- wherein said nucleotide sequence encodes a P-glycoprotein that controls plant height or said nucleotide sequence is complementary to a nucleotide sequence that encodes said P-glycoprotein.
An expression cassette comprising a nucleic acid molecule operably linked to a promoter that drives expression in a plant cell, wherein said nucleic acid molecule comprises a nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence having at least 95% nucleotide sequence identity to the nucleotide sequence set forth in SEQ ID NO:
- 1;
- (b) a nucleotide sequence having at least 95% nucleotide sequence identity to the nucleotide sequence set forth in SEQ ID NO:
- 2;
- (c) a nucleotide sequence encoding a variant of the amino acid sequence set forth in SEQ ID NO:
- 3, wherein said variant has at least 90% amino acid sequence identity to the amino acid sequence set forth in SEQ ID NO:
- 3;
- and
- (d) a nucleotide sequence that is fully complementary to the nucleotide sequence of any one of (a)-(c);
- wherein said nucleotide sequence encodes a P-glycoprotein that controls plant height or said nucleotide sequence is complementary to a nucleotide sequence that encodes said P-glycoprotein.
A transformed plant cell having stably incorporated into its genome a nucleic acid molecule operably linked to a promoter that drives expression in a plant cell, wherein said nucleic acid molecule comprises a nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence having at least 95% nucleotide sequence identity to the nucleotide sequence set forth in SEQ ID NO:
- 1;
- (b) a nucleotide sequence having at least 95% nucleotide sequence identity to the nucleotide sequence set forth in SEQ ID NO:
- 2;
- (c) a nucleotide sequence encoding a variant of the amino acid sequence set forth in SEQ ID NO:
- 3, wherein said variant has at least 90% amino acid sequence identity to the amino acid sequence set forth in SEQ ID NO:
- 3;
- and
- (d) a nucleotide sequence that is fully complementary to the nucleotide sequence of any one of (a)-(c);
- wherein said nucleotide sequence encodes a P-glycoprotein that controls plant height or said nucleotide sequence is complementary to a nucleotide sequence that encodes said P-glycoprotein.
A transformed plant having stably incorporated into its genome a nucleic acid molecule operably linked to a promoter that drives expression in a plant cell, wherein said nucleic acid molecule comprises a nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence having at least 95% nucleotide sequence identity to the nucleotide sequence set forth in SEQ ID NO:
- 1;
- (b) a nucleotide sequence having at least 95% nucleotide sequence identity to the nucleotide sequence set forth in SEQ ID NO:
- 2;
- (c) a nucleotide sequence encoding a variant of the amino acid sequence set forth in SEQ ID NO:
- 3, wherein said variant has at least 90% amino acid sequence identity to the amino acid sequence set forth in SEQ ID NO:
- 3;
- and
- (d) a nucleotide sequence that is fully complementary to the nucleotide sequence of any one of (a)-(c);
- wherein said nucleotide sequence encodes a P-glycoprotein that controls plant height or said nucleotide sequence is complementary to a nucleotide sequence that encodes said P-glycoprotein.
A transformed seed of a transformed plant, said transformed plant having stably incorporated into its genome a nucleic acid molecule operably linked to a promoter that drives expression in a plant cell, wherein said nucleic acid molecule comprises a nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence having at least 95% nucleotide sequence identity to the nucleotide sequence set forth in SEQ ID NO:
- 1;
- (b) a nucleotide sequence having at least 95% nucleotide sequence identity to the nucleotide sequence set forth in SEQ ID NO:
- 2;
- (c) a nucleotide sequence encoding a variant of the amino acid sequence set forth in SEQ ID NO:
- 3, wherein said variant has at least 90% amino acid sequence identity to the amino acid sequence set forth in SEQ ID NO:
- 3;
- and
- (d) a nucleotide sequence that is fully complementary to the nucleotide sequence of any one of (a)-(c);
- wherein said nucleotide sequence encodes a P-glycoprotein that controls plant height or said nucleotide sequence is complementary to a nucleotide sequence that encodes said P-glycoprotein and wherein said seed comprises said nucleic acid molecule operably linked to said promoter.
A method for modifying the growth of a plant, said method comprising transforming a plant with a nucleic acid molecule operably linked to a promoter that drives the expression of said nucleic acid molecule in said plant, wherein said nucleic acid molecule comprises a nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence having at least 95% nucleotide sequence identity to the nucleotide sequence set forth in SEQ ID NO:
- 1;
- (b) a nucleotide sequence having at least 95% nucleotide sequence identity to the nucleotide sequence set forth in SEQ ID NO:
- 2;
- (c) a nucleotide sequence encoding a variant of the amino acid sequence set forth in SEQ ID NO:
- 3, wherein said variant has at least 90% amino acid sequence identity to the amino acid sequence set forth in SEQ ID NO:
- 3;
- and
- (d) a nucleotide sequence that is fully complementary to the nucleotide sequence of any one of (a)-(c);
- wherein said nucleotide sequence encodes a P-glycoprotein that controls plant height or said nucleotide sequence is complementary to a nucleotide sequence that encodes said P-glycoprotein and wherein the height of the transformed plant is reduced or increased when compared to an untransformed plant.
Dependencies are inferred from the claim text.