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AssigneeMonsanto Technology LLC
InventorsScott E. Andersen, Glenn R. Hicks, Joseph E. Huesing, Charles P. Romano, Clayton S. Vetsch
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Expressed Sequence Tags (ESTs) isolated from the Western Corn Rootworm, Diabrotica virgifera virgifera LeConte, are disclosed. The invention encompasses nucleic acid molecules that encode D. v. virgifera protein homologs and fragments thereof. In addition, antibodies capable of binding the proteins are encompassed by the present invention. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The invention also relates to methods of using the disclosed nucleic acid molecules, proteins, fragments of proteins, and antibodies, for example, for gene identification and analysis, and preparation of constructs.
A nucleic acid molecule complementary to all or part of a sequence selected from the group consisting of SEQ ID NO:1684, SEQ ID NO:1685, SEQ ID NO:1686 and SEQ ID NO:1687, wherein the expression of the nucleic acid molecule as antisense RNA blocks the expression of D. v. virgifera V-ATPASE, and wherein the nucleic acid molecule hybridizes to a sequence selected from the group consisting of SEQ ID NO:1684, SEQ ID NO:1685, SEQ ID NO:1686 and SEQ ID NO:1687 under wash conditions of 0.2×SSC at 65° C., and wherein the nucleic acid molecule is operably linked to a heterologous promoter that causes transcription of DNA in plant cells.
Dependencies are inferred from the claim text.