Cell cycle phase markers
AssigneesGe Healthcare UK Limited, Vanderbilt University
InventorsSuzanne Hancock, Simon Stubbs, Nicholas Thomas, Ellen Fanning, Jinming Gu
Life of this patent
Estimating the term…
Abstract
The present invention relates to polypeptide and nucleic acids constructs which are useful for determining the cell cycle status of a mammalian cell. Host cells transfected with these nucleic acid constructs can be used to determine the effects that test agents have upon the mammalian cell cycle.
Claims9
An isolated polypeptide construct comprising a detectable live-cell reporter molecule linked via a polypeptide group having a molecular mass of less than 1,000 Daltons to another polypeptide consisting of amino acid sequence 957-1087 of SEQ ID NO:1, which amino acid sequence is the phosphorylation-dependent subcellular localization domain of the C-terminal special control region of helicase B (PSLD), wherein the translocation of said construct within a mammalian cell is indicative of the cell cycle position.
An isolated polypeptide construct comprising the amino acid sequence of SEQ ID NO. 5.
Dependencies are inferred from the claim text.