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AssigneeThe Salk Institute for Biological Studies
InventorsTarmo Roosild, Jason Greenwald, Senyon Choe, Roland Riek
Estimating the term…
A class of integral membrane proteins, referred to as Mistic polypeptides, their variants, fusion proteins including a Mistic polypeptide domain, and nucleic acid molecules encoding Mistic polypeptides and Mistic fusion proteins are disclosed herein. Also described are methods of using Mistic polypeptides and Mistic fusion proteins to produce and/or isolate recombinant proteins (including without limitation classes of eukaryotic proteins that have previously been intractable to recombinant bacterial expression, such as, eukaryotic integral membrane proteins).
An isolated nucleic acid molecule encoding a polypeptide of no more than 125 amino acids in length comprising:
An isolated nucleic acid encoding a polypeptide, wherein the polypeptide has the tertiary structure of the atomic structure coordinates set forth in PDB Accession No. 1YGM as disclosed in Table 4.
An isolated nucleic acid molecule encoding a fusion protein comprising a cargo protein domain and a Mistic domain;
A vector comprising a promoter sequence operably linked to an isolated nucleic acid molecule encoding a fusion protein comprising a cargo protein domain and a Mistic domain;
An isolated nucleic acid molecule encoding a polypeptide of no more than 125 amino acids in length wherein the nucleic acid molecule encodes an amino acid sequence comprising SEQ ID NO:
An isolated nucleic acid molecule encoding a fusion protein comprising a cargo protein domain and a Mistic domain;
Dependencies are inferred from the claim text.