Back to results
US 7,612,185 B2Granted
Nucleic acid biosensors
AssigneeThe Board of Trustees of the University of Illinois
InventorsYi Lu, Juewen Liu
Life of this patent
Estimating the term…
Abstract
Sensors comprising aptazymes capable of detecting the presence and concentration of effectors, as well as methods of using such sensors, are disclosed.
Claims20
A sensor system for detecting an effector, comprising:
- (i) an aggregate, wherein the aggregate comprises a plurality of complexes wherein each complex comprises:
- (I) an aptazyme, comprising an aptamer comprising a binding site for the effector,
- (II) a substrate for the aptazyme, hybridized to the aptazyme,
- (III) particles,
- (IV) polynucleotides attached to the particles at the 5′ terminus or 3′ terminus and hybridized to the substrate,
- wherein the aptazyme cleaves the substrate when the aptamer binds the effector and cleavage of the substrate results in deaggregation of the aggregate.
3 more elements
A sensor system for detecting an effector, comprising:
- (i) an aggregate, wherein the aggregate comprises a plurality of complexes wherein each complex comprises:
- (I) an aptazyme, comprising an aptamer comprising a binding site for the effector,
- (II) a substrate for the aptazyme, hybridized to the aptazyme,
- (III) gold particles,
- (IV) polynucleotides attached to the particles at the 5′-terminus or 3′-terminus, and hybridized to the substrate, and
- (ii) Mg(II),
- wherein the aptazyme comprises DNA,
- the aptazyme cleaves the substrate when the aptamer binds the effector and cleavage of the substrate results in deaggregation of the aggregate.
5 more elements
A sensor system for detecting an effector, comprising:
- (i) an aggregate, wherein the aggregate comprises a plurality of complexes wherein each complex comprises:
- (I) an aptazyme, comprising an aptamer comprising a binding site for the effector,
- (II) a substrate for the aptazyme, hybridized to the aptazyme,
- (III) first particles,
- (IV) first polynucleotides attached to the first particles at the 5′-terminus or 3′-terminus and hybridized to the substrate;
- (V) second particles,
- (VI) second polynucleotides attached to the second particles at the 5′-terminus or 3′-terminus and hybridized to the substrate,
- wherein the aptazyme cleaves the substrate when the aptamer binds the effector and cleavage of the substrate results in deaggregation of the aggregate.
5 more elements
Dependencies are inferred from the claim text.