Solid-phase synthesis is a capillary
AssigneePhyNexus, Inc.
InventorDouglas T. Gjerde
Life of this patent
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Abstract
The invention provides, inter alia, methods for synthesizing a molecule on the channel surface of a capillary, comprising the steps of: (i) covalently attaching a first chemical entity to the channel surface of a capillary; and (ii) covalently attaching a second chemical entity to the first chemical entity, wherein the covalent attachment steps are part of a process for synthesizing a molecule on the channel surface.
Claims20
A method of synthesizing a biopolymer on the internal surface of a capillary, comprising the steps of:
- (i) covalently attaching a first chemical entity to the internal surface of a capillary;
- and
- (ii) covalently attaching a second chemical entity to the first chemical entity,
- (iii) optionally repeating the attachment iteratively with the attachment of additional chemical entities, wherein the covalent attachment steps are part of a process for synthesizing the biopolymer on the internal surface of the capillary, and
- wherein the capillary has an internal diameter from a lower limit of 20 μm to an upper limit of 1 mm and a length greater than 1 cm and less than 5 m.
A method of synthesizing a biopolymer on the internal surface of a capillary, comprising the steps of:
- (i) covalently attaching a first chemical entity to the internal surface of a capillary;
- and
- (ii) covalently attaching a second chemical entity to the first chemical entity,
- (iii) optionally repeating the attachment iteratively with the attachment of additional chemical entities, wherein the covalent attachment steps are part of a process for synthesizing the biopolymer on the internal surface of the capillary,
- (iv) optionally removing substantially all liquid from the capillary;
- and
- (v) passing a slug of cleavage solution through the capillary to cleave the synthesized biopolymer off the internal surface of the capillary,
- wherein the capillary has an internal diameter from a lower limit of 20 μm to an upper limit of 1 mm and a length greater than 1 cm and less than 5 m, and
- wherein the slug of cleavage solution has a volume less than the total capillary volume.
A method of synthesizing biopolymers on the internal surface of a plurality of capillaries, comprising the steps of:
- (i) covalently attaching a first chemical entity to the internal surface of each capillary;
- and
- (ii) covalently attaching a second chemical entity to the first chemical entity,
- (iii) optionally repeating the attachment iteratively with the attachment of additional chemical entities, wherein the covalent attachment steps are part of a process for synthesizing the biopolymers on the internal surface of the capillaries,
- (iv) optionally removing substantially all liquid from the capillaries;
- and
- (v) passing a slug of cleavage solution through the capillaries to cleave the synthesized biopolymer off the internal surface of the capillaries,
- wherein each capillary has an internal diameter from a lower limit of 20 μm to an upper limit of 1 mm and a length greater than 1 cm and less than 5 m, wherein each capillary is addressable and wherein the slug of cleavage solution has a volume less than the total capillary volume.
Dependencies are inferred from the claim text.