Supramolecular complexes as photoactivated DNA cleavage agents
AssigneeVirginia Tech Intellectual Properties, Inc.
InventorsKaren Brewer, Shawn Swavey
Life of this patent
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Abstract
The invention provides supramolecular metal complexes as DNA cleaving agents. In the complexes, charge is transferred from one light absorbing metal (e.g. Ru or Os) to an electron accepting metal (e.g. Rh) via a bridging π-acceptor ligand. A bioactive metal-to-metal charge transfer state capable of cleaving DNA is thus generated. The complexes function when irradiated with low energy visible light with or without molecular oxygen.
Claims8
A method for cleaving DNA comprising the steps of combining said DNA with a supramolecular complex selected from the group consisting of:
- [(2,2′-bipyridine)2Ru(2-pyridyl)pyrazine)RhCl2(2-pyridyl)pyrazine)Ru(2,2′-bipyridine)2](PF6)5;
- [(2,2′-bipyridine)2Os(2,3-bis(2-pyridyl)pyrazine)RhCl2(2,3-bis(2-pyridyl)pyrazine)Os(2,2′-bipyridine)2](PF6)5;
- [(2,2′:6′,2″-terpyridine)RuCl(2,3-bis(2-pyridyl)pyrazine)RhCl2(2,3-bis(2-pyridyl)pyrazine)RuCl(2,2′:6′,2″-terpyridine)](PF6)3;
- and
- [(2,2′:6′,2″-terpyridine)RuCl(2,2′-bipyridimidine)RhCl2(2,2′-bipyridimidine)RuCl(2,2′:6′,2″-terpyridine)](PF6)3.
- wherein said supramolecular complex is present in sufficient quantity to bind to and cleave said DNA;
- and
- exposing said DNA to light or radiant energy sufficient to activate said supramolecular complex to cleave said DNA.
A method for cleaving DNA comprising the steps of:
- delivering to said DNA a supramolecular complex selected from the group consisting of:
- [(2,2′-bipyridine)2Ru(2-pyridyl)pyrazine)RhCl2(2-pyridyl)pyrazine)Ru(2,2′-bipyridine)2](PF6)5;
- [(2,2′-bipyridine)2Os(2,3-bis(2-pyridyl)pyrazine)RhCl2(2,3-bis(2-pyridyl)pyrazine)Os(2,2′-bipyridine)2](PF6)5;
- [(2,2′:6′,2″-terpyridine)RuCl(2,3-bis(2-pyridyl)pyrazine)RhCl2(2,3-bis(2-pyridyl)pyrazine)RuCl(2,2′:6′,2″-terpyridine)](PF6)3;
- [(2,2′:6′,2″-terpyridine)RuCl(2,2′-bipyridimidine)RhCl2(2,2′-bipyridimidine)RuCl(2,2′:6′,2″-terpyridine)](PF6)3;
- and
- pharmaceutically acceptable salts thereof, and
- applying light or radiant energy to said DNA and said supramolecular complex,
- wherein said step of applying light induces the production of a metal-to-metal charge transfer state within said supramolecular complex, and wherein said metal-to-metal charge transfer state mediates the cleavage of said DNA.
Dependencies are inferred from the claim text.