Enzymatic production of peracids using perhydrolytic enzymes

A process for producing a concentrated aqueous peracid solution comprising;

• a) providing a set of peracid reaction components, said components comprising:
• 1) at least one substrate selected from the group consisting of:
• i) esters having the structure
• wherein R1=C1 to C10 straight chain or branched chain alkyl optionally substituted with an hydroxyl or a C1 to C4 alkoxy group and R2=C1 to C10 straight chain or branched chain alkyl group, (CH2CH2—O)nH or (CH2CH(CH3)—O)nH and n=1 to 10;
• ii) glycerides having the structure
• wherein R1=C1 to C10 straight chain or branched chain alkyl optionally substituted with an hydroxyl or a C1 to C4 alkoxy group and R3 and R4 are individually H or R1C(O);
• and
• iii) amides having the structure
• wherein R5 and R6are individually H, acetyl, or a C1 to C5 straight chain or branched alkyl group;
• 2) a source of peroxygen that provides a concentration of at least 500 mM hydrogen peroxide upon combining said reaction components;
• and
• 3) at least one enzyme catalyst having perhydrolase activity, wherein said enzyme catalyst is selected from the group consisting of lipases, esterases, proteases, and mixtures thereof;
• and
• b) combining said reaction components at a pH of about 2.5 to about 7.5, whereby a concentrated peracid solution is produced within at least about 5 minutes to about 2 hours after combining said reaction components.