Agrobacterium transformation of stolons
AssigneeThe Samuel Roberts Noble Foundation
InventorsZengyu Wang, Yaxin Ge
Life of this patent
Estimating the term…
Abstract
The invention provides methods for transforming monocotyledonous plants with Agrobacterium, for example, using stolons as a target tissue. The invention allows creation of transgenic plants without the need for callus as a target tissue for transformation, thus providing a rapid method for the production of transgenic plants. The ability to directly regenerate transgenic plants from stolons significantly reduces the time, labor and other complications required to produce transgenic plants. Plants produced by such methods are also provided by the invention.
Claims16
A method of transforming a stolon-producing turfgrass plant comprising contacting a stolon of the plant with an Agrobacterium comprising a recombinant DNA and selecting a transformed tissue comprising the recombinant DNA, wherein the stolon is contacted with the Agrobacterium without first forming a callus culture of cells from the stolon.
Dependencies are inferred from the claim text.