AP1 amine oxidase variants
AssigneePioneer Hi-Bred International, Inc.
InventorsRanjini Chatterjee, Jonathan P. Duvick, James English
Life of this patent
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Abstract
New fumonisin detoxifying or fumonisin-derivative detoxifying homologues (both nucleic acids and proteins) are provided. Compositions which include these new proteins, recombinant cells, antibodies to the new homologues, and methods of using the homologues are also provided.
Claims25
An isolated or recombinant nucleic acid comprising a polynucleotide sequence that encodes a polypeptide that is at least 97% identical to the entire length of SEQ ID NO:50 or a fully complementary polynucleotide sequence thereof, wherein at pH 5.5 said polypeptide has a fumonisin detoxification activity or a fumonisin derivative detoxification activity that is at least 1.5-fold greater than any of the polypeptides selected from the group consisting of:
- SEQ ID NO:54;
- SEQ ID NO:56;
- SEQ ID NO:58;
- SEQ ID NO:60;
- SEQ ID NO:62;
- SEQ ID NO:64;
- SEQ ID NO:5 land SEQ ID NO:52.
An isolated or recombinant nucleic acid comprising a polynucleotide sequence selected from the group consisting of:
- (a) a polynucleotide sequence selected from the group consisting of SEQ ID NOs:
- 21-25, SEQ ID NOs:
- 67-68, and SEQ ID NOs:
- 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, and 126;
- (b) a polynucleotide sequence encoding a polypeptide selected from the group consisting of SEQ ID NOs:
- 46-50, SEQ ID NO:
- 71, and SEQ ID NOs:
- 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, and 127,
- wherein at pH 5.5 said polypeptide has a fumonisin detoxification activity or a fumonisin derivative detoxification activity that is at least 1.5-fold greater than any of the polypeptides selected from the group consisting of:
- SEQ ID NO:
- 54;
- SEQ ID NO:
- 56;
- SEQ ID NO:
- 58;
- SEQ ID NO:
- 60;
- SEQ ID NO:
- 62;
- SEQ ID NO:
- 64;
- SEQ ID NO:
- 51 and SEQ ID NO:
- 52;
- and
- (c) a polynucleotide sequence fully complementary to the polynucleotide of (a) or (b).
Dependencies are inferred from the claim text.