Rotaxane compound, rotaxane-bonded solid substrate, and biochip using the same

A solid substrate for a biochip comprising a compound represented by Formula 1 below:

• wherein R1, R2, and R3 are each independently saturated or unsaturated linear C2-C10 alkylene, ethyleneglycol oligomer, 1,4-substituted benzene, or 1,4-substituted pyridine;
• R4 and R5 are each independently hydrogen, an alkenyloxy group with an unsaturated bond end and a substituted or unsubstituted alkyl moiety of C1-C20, a carboxyalkylsulfinyloxy group with a substituted or unsubstituted alkyl moiety of C1-C20, a carboxyalkyloxy group with a substituted or unsubstituted alkyl moiety of C2-C8, an aminoalkyloxy group with a substituted or unsubstituted alkyl moiety of C2-C8, or a hydroxyalkyloxy group with a substituted or unsubstituted alkyl moiety of C2-C8;
• X1 and X2 are each independently a positively charged functional group for ion-dipole interaction with an oxygen atom of cucurbituril or its derivative of represented by Formula 2;
• Y1 is a functional group for a linkage with a biomaterial comprising a gene or a protein;
• and Y2 is a functional group capable of binding to the solid substrate, wherein the compound of Formula 1 bonded to the solid substrate provides a linkage layer with a predetermined spacing in the biochip;
• and
• wherein a compound of Formula 3
• Y1—R1—X1—R2—X2—R3—Y2 (3)
• (wherein R1, R2, R3, X1, X2, Y1, and Y2 are as defined in Formula 1 above)
• vertically passes through a cavity of cucurbituril or its derivative represented by Formula 2
• (wherein n is an integer of 4 to 20;
• and R4 and R5 are as defined in Formula 1 above).