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US 7,611,883 B2Granted
Plasmid maintenance
AssigneeCobra Biologics Limited
InventorRocky Marc Cranenburgh
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Abstract
The invention relates to a system for stable maintenance of a plasmid, to host cells for use in this system and to methods of using the system to obtain a plasmid useful in medical applications. In particular, the invention provides transformed host cell containing: i) a chromosomal gene which inhibits cell growth; and ii) a plasmid encoding an antisense sequence, wherein the antisense sequence encoded by the plasmid inhibits the action of the chromosomal gene, thereby permitting cell growth and a method for stable maintenance of a plasmid in a host cell in vivo.
Claims37
An isolated transformed host cell comprising
- i) a chromosomal gene which inhibits cell growth operably linked to a regulatory sequence;
- and
- ii) a plasmid comprising an origin of replication encoding an antisense sequence which binds to mRNA transcribed from the regulatory sequence,
- wherein the binding of the antisense sequence encoded by the origin of replication of the plasmid to mRNA transcribed from the regulatory sequence inhibits the action of the chromosomal gene, thereby permitting cell growth.
1 more element
Dependencies are inferred from the claim text.