PUFA polyketide synthase systems and uses thereof
AssigneeMartek Biosciences Corporation
InventorsJames G. Metz, Craig A. Weaver, William R. Barclay, James H. Flatt
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Abstract
The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.
Claims16
A genetically modified microorganism, wherein the microorganism has been transfected with at least one recombinant nucleic acid molecule encoding a polyketide synthase (PKS) system for the production of polyunsaturated fatty acids (PUFAs), wherein the PKS system comprises the following proteins:
- a) a protein comprising an amino acid sequence that is at least 95% identical to SEQ ID NO:39 and has β-keto acyl-ACP synthase (KS) activity, malonyl-CoA:ACP acyltransferase (MAT) activity, acyl carrier protein (ACP) activity and ketoreductase (KR) activity;
- b) a protein comprising an amino acid sequence that is at least 95% identical to SEQ ID NO:52 and has β-keto acyl-ACP synthase (KS) activity, chain length factor (CLF) activity, acyl transferase (AT) activity, and enoyl ACP-reductase (ER) activity;
- and
- c) a protein comprising an amino acid sequence that is at least 95% identical to SEQ ID NO:62 and has FabA-like β-hydroxy acyl-ACP dehydrase (DH) activity and enoyl ACP-reductase (ER) activity.
Dependencies are inferred from the claim text.