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AssigneeNitto Boseki Co., Ltd.
InventorsTatsuya Ohashi, Toshihide Miura, Yoshihiko Igarashi, Fumio Nomura, Takeshi Tomonaga, Katsuhiro Katayama
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Using two types of antibodies, i.e., a first antibody having a higher affinity for a target substance than for a competitive substance and a second antibody having a higher affinity for the competitive substance than for the target substance, a specimen is treated with these two antibodies. Then, the competitive substance in the specimen first binds to the second antibody and thus the ratio of the target substance to the competitive substance in the specimen is enlarged. As a result, the target substance becomes liable to bind to the first antibody and, in its turn, the reactivity of the target substance is elevated compared with the case of using the first antibody alone. Thus, the target substance in the specimen can be accurately assayed while avoiding the effects of the competitive substance contained in the specimen.
An immunoassay method in which tartrate resistant acid phosphatase 5b (TRACP 5b) is assayed in a specimen containing the tartrate resistant acid phosphatase 5b (TRACP 5b) together with a competitive substance therein, the method comprising:
A kit for immunoassay of a target substance in a specimen by the use of two types of antibodies, which comprises
Dependencies are inferred from the claim text.