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US 7,611,834 B2Granted
Methods and devices for protein assays
AssigneeSandia Corporation
InventorsSwapnil Chhabra, José´ M. Cintrón, Renée Shediac
Life of this patent
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Abstract
Methods and devices for protein assays based on Edman degradation in microfluidic channels are disclosed herein. As disclosed, the cleaved amino acid residues may be immobilized in an array format and identified by detectable labels, such as antibodies, which specifically bind given amino acid residues. Alternatively, the antibodies are immobilized in an array format and the cleaved amino acids are labeled identified by being bound by the antibodies in the array.
Claims12
An assay comprising
- subjecting a protein to consecutive coupling and cleaving steps to result in a plurality of cleaved amino acid residues in a microfluidic device,
- contacting each cleaved amino acid residue with a plurality of antibodies, which each antibody specifically binds a given amino acid,
- identifying each cleaved amino acid residue by the antibody bound thereto, and
- identifying the location of each cleaved amino acid residue in the sequence of the protein.
1 more element
A microfluidic device comprising
- at least one microchannel having an amino acid residue bound to an antibody that specifically binds the amino acid residue and said antibody is conjugated to a detectable label.
Dependencies are inferred from the claim text.