Device to lavage a blood vessel
AssigneeThe United States of America as represented by the Department of Veterans Affairs
InventorsHemant Thatte, Kunda Biswas, Shukri Khuri, Thomas Michel
Life of this patent
Estimating the term…
Abstract
The present invention provides for compositions and methods for the preservation of tissues and organs ex vivo and in situ. In addition, the present invention provides for kits that may be used in the preparation of the solutions of the present invention. The present invention also provides a device for perfusing tissues and organs with the solutions of the present invention.
Claims13
A device comprising, an incomplete, hollow circuit defining a liquid flow path in fluidic communication with a chamber comprising an inlet port and an outlet port, said incomplete circuit comprising a pump operably linked to a pressure transducer, said circuit terminating at first and second attachment points, wherein said circuit comprises an efflux port disposed between said inlet port and said first attachment point and an uptake port disposed between said outlet port and said second attachment point, wherein the bore of said uptake port bore is larger than the bore of said efflux port thereby resulting in a bore size differential creating enough turbulence to continuously lavage a blood vessel, such that attachment of a first end of said blood vessel to said first attachment point and a second end of said blood vessel to said second attachment point generates a complete, hollow circuit defining a liquid flow path.
A method for perfusion, comprising:
- a) providing i) a device comprising, an incomplete, hollow circuit defining a liquid flow path in fluidic communication with a chamber comprising an inlet port and an outlet port, said incomplete circuit comprising a pump operably linked to a pressure transducer, said circuit terminating at first and second attachment points, wherein said circuit comprises an efflux port disposed between said inlet port and said first attachment point and an uptake port disposed between said outlet port and said second attachment point, wherein the bore of said uptake port bore is larger than the bore of said efflux port thereby resulting in a bore size differential creating enough turbulence to continuously lavage a blood vessel;
- and ii) a segment of a blood vessel, said blood vessel having a first end and a second end;
- b) attaching, in any order, said first end of said segment to said first attachment point and said second end of said segment to said second attachment point, under conditions such that a complete, hollow circuit defining a liquid flow path is produced;
- and
- c) circulating an aqueous solution in said hollow circuit.
Dependencies are inferred from the claim text.