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US 7,611,718 B1Granted
Attenuation of cytomegalovirus virulence
AssigneeMedImmune, LLC
InventorsGeorge Kemble, Gregory M. Duke, Richard Spaete
Life of this patent
Estimating the term…
Abstract
A method is provided for attenuating a cytomegalovirus comprising functionally disrupting an open reading frame of a Toledo genome region or its homolog and making chimeric CMV virus genomes.
Claims6
A chimeric cytomegalovirus (CMV) virus which comprises:
- (a) the polynucleotide sequence of a high-passage Towne genome from nucleotides 1 to 99000;
- (b) the polynucleotide sequence of a Toledo genome from nucleotides 108094 to 203136;
- (c) the polynucleotide sequence of a high passage Towne genome from nucleotides 205803 to S-term, wherein the high-passage Towne genome has been passaged at least 50 times and wherein the nucleotide number of the Towne and Toledo genomes is according to the numbering convention of the AD169 genome.
A chimeric CMV virus which comprises:
- (a) the polynucleotide sequence of a high-passage Towne genome from nucleotides 1 to 99000;
- (b) a crossover region comprising SEQ ID NO.:
- 29;
- (c) the polynucleotide sequence of a Toledo genome from nucleotides 108094 to 203136;
- (d) a crossover region comprising SEQ ID NO.:
- 26;
- (e) the polynucleotide sequence of a high passage Towne genome from nucleotides 205803 to S-term, wherein the high-passage Towne genome has been passaged at least 50 times and wherein the nucleotide number of the Towne and Toledo genomes is according to the numbering convention of the AD169 genome.
4 more elements
Dependencies are inferred from the claim text.