Promoters for epidermis-specific expression of desired coding sequences in transgenic plants
AssigneesIPK-Institut Fur Pflanzengenetik Und Kulturpflanzenforschung, Max-Planck-Gesellschaft zur Forderung der Wissenschaften e.V., UniversitäT ZüRich
InventorsPatrick Schweizer, Robert Dudler, Paul Schulze-Lefert, Ralph Panstruga
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Abstract
The invention relates to methods of producing transgenic plants including pathogen resistant plants with recombinant nucleic acid molecules comprising promoter regions which provides expression of a desired transgene in epidermis-specific manner, and transgenic plants produced by said methods.
Claims17
A method of causing epidermis specific expression of a desired coding sequence in a transgenic plant, the method comprising introducing into a plant cell a chimeric gene comprising an isolated promoter region that controls epidermis-specific expression operably linked to the desired coding sequence, said promoter region comprising a first sequence originating from the promoter of a GSTA1 gene and a second sequence originating from the intron of a WIR1a gene, wherein the first sequence is SEQ ID No. 1 and the second sequence is SEQ ID No. 2 or wherein the first sequence has at least 95% sequence identity to SEQ ID No. 1 and the second sequence has at least 95% sequence identity to SEQ ID No. 2.
A method for increasing pathogen resistance in a plant, the method comprising transforming a plant cell with a chimeric gene comprising an isolated promoter region that controls epidermis-specific expression operably linked to a DNA encoding a protein that confers pathogen resistance, said promoter region comprising a first sequence originating from the promoter of a GSTA1 gene and a second sequence originating from the intron of a WIR1a gene, wherein the first sequence is SEQ ID No. 1 and the second sequence is SEQ ID No. 2 or wherein the first sequence has at least 95% sequence identity to SEQ ID No. 1 and the second sequence has at least 95% sequence identity to SEQ ID No. 2;
- and regenerating a transformed plant from the transformed plant cell;
- said transformed plant exhibits increased resistance to a pathogen.
Dependencies are inferred from the claim text.