PUFA polyketide synthase systems and uses thereof
AssigneeMartek Biosciences Corporation
InventorsJames G. Metz, Craig A. Weaver, William R. Barclay, James H. Flatt
Life of this patent
Estimating the term…
Abstract
The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.
Claims21
A genetically modified plant, wherein cells of the plant are transfected with at least one recombinant nucleic acid molecule encoding a polyketide synthase (PKS) system for the production of polyunsaturated fatty acids (PUFAs), wherein the PKS system comprises the following proteins:
- a) a protein comprising an amino acid sequence that is at least 95% identical to SEQ ID NO:39 and has β-keto acyl-ACP synthase (KS) activity, malonyl-CoA:ACP acyltransferase (MAT) activity, acyl carrier protein (ACP) activity and ketoreductase (KR) activity;
- b) a protein comprising an amino acid sequence that is at least 95% identical to SEQ ID NO:52 and has β-keto acyl-ACP synthase (KS) activity, chain length factor (CLF) activity, acyl transferase (AT) activity, and enoyl ACP-reductase (ER) activity;
- and
- c) a protein comprising an amino acid sequence that is at least 95% identical to SEQ ID NO:62 and has FabA-like β-hydroxy acyl-ACP dehydrase (DH) activity and enoyl ACP-reductase (ER) activity.
A recombinant plant cell, wherein the plant cell expresses at least one recombinant nucleic acid molecule encoding a polyketide synthase (PKS) system for the production of polyunsaturated fatty acids (PUFAs), wherein the PKS system comprises the following proteins:
- a) a protein comprising an amino acid sequence that is at least 95% identical to SEQ ID NO:39 and has β-keto acyl-ACP synthase (KS) activity, malonyl-CoA:ACP acyltransferase (MAT) activity, acyl carrier protein (ACP) activity and ketoreductase (KR) activity;
- b) a protein comprising an amino acid sequence that is at least 95% identical to SEQ ID NO:52 and has β-keto acyl-ACP synthase (KS) activity, chain length factor (CLF) activity, acyl transferase (AT) activity, and enoyl ACP-reductase (ER) activity;
- and
- c) a protein comprising an amino acid sequence that is at least 95% identical to SEQ ID NO:62 and has FabA-like β-hydroxy acyl-ACP dehydrase (DH) activity and enoyl ACP-reductase (ER) activity.
A method to produce a genetically modified plant that has a polyunsaturated fatty acid (PUFA) profile that differs from the naturally occurring plant and that produces at least one long chain polyunsaturated fatty acid (PUFA), comprising transfecting plant cells with at least one recombinant nucleic acid molecule encoding a polyketide synthase (PKS) system for the production of polyunsaturated fatty acids (PUFAs) and producing the plant using the plant cells, wherein the PKS system comprises the following proteins:
- a) a protein comprising an amino acid sequence that is at least 95% identical to SEQ ID NO39 and has 13-keto acyl-ACP synthase (KS) activity, malonyl-CoA:ACP acyltransferase (MAT) activity, acyl carrier protein (ACP) activity and ketoreductase (KR) activity;
- b) a protein comprising an amino acid sequence that is at least 95% identical to SEQ ID NO:52 and has 13-keto acyl-ACP synthase (KS) activity, chain length factor (CLF) activity, acyl transferase (AT) activity, and enoyl ACP-reductase (ER) activity;
- and
- c) a protein comprising an amino acid sequence that is at least 95% identical to SEQ ID NO:62 and has FabA-like β-hydroxy acyl-ACP dehydrase (DH) activity and enoyl ACP-reductase (ER) activity.
Dependencies are inferred from the claim text.