Soluble low-density lipoprotein receptor related protein binds directly to Alzheimer's amyloid-beta peptide
AssigneesUniversity of Rochester, Socratech L.L.C.
InventorsBerislav V. Zlokovic, Rashid Deane
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Abstract
A soluble derivative of low-density lipoprotein receptor related protein-1 (sLRP-1) binds directly to Alzheimer's amyloid-β peptide (Aβ). This binding may be used to detect Aβ or to separate Aβ from the rest of a subject's body. In Alzheimer's disease, it may be used to provide diagnostic results by detecting Aβ, treatment by removing Aβ, or both.
Claims40
A method of binding amyloid-β peptide (Aβ) in a body fluid and/or tissue of a subject, said method comprising:
- (a) providing a soluble derivative of low-density lipoprotein receptor related protein-1 (LRP-1), wherein said soluble LRP-1 derivative is comprised of cluster II, cluster IV, or both;
- (b) contacting said soluble LRP-1 derivative with at least said body fluid and/or tissue of said subject such that said Aβ is specifically bound;
- and
- (c) detecting said soluble LRP-1 derivative bound to Aβ.
A method of binding amyioid-β peptide (Aβ) in a body fluid of a human subject, said method comprising:
- (a) providing a soluble fragment of low-density lipoprotein receptor related protein-1 (LRP-1) comprising cluster II, cluster IV, or both of human LRP-1;
- (b) contacting said soluble fragment of LRP-1 with at least said body fluid of said human subject such that said Aβ is specifically bound;
- and
- (c) detecting said soluble fragment of LRP-1 bound to Aβ.
A method of binding amyloid-β peptide (Aβ) in a body fluid and/or tissue of a subject, said method comprising:
- (a) providing a soluble derivative of low-density lipoprotein receptor related protein-1 (LRP-1), wherein said soluble LRP-1 derivative is comprised of cluster II, cluster IV, or both;
- (b) contacting said soluble LRP-1 derivative with at least said body fluid and/or tissue of said subject such that said Aβ is specifically bound;
- and
- (c) removing said soluble LRP-1 derivative bound to Aβ from said subject's body.
A method of binding amyloid-β peptide (Aβ) in a body fluid of a human subject, said method comprising:
- (a) providing a soluble fragment of low-density lipoprotein receptor related protein-1 (LRP-1) comprising cluster II, cluster IV, or both of human LRP-1;
- (b) contacting said soluble fragment of LRP-1 with at least said body fluid of said human subject such that said Aβ is specifically bound;
- and
- (c) removing said soluble fragment of LRP-1 derivative bound to Aβ from said subject's body.
Dependencies are inferred from the claim text.