Modified reconstituted basement membrane composition for assay system
AssigneeBecton, Dickinson and Company
InventorsMin Wu, Frank J. Mannuzza
Life of this patent
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Abstract
A modified reconstituted extracellular matrix composition is provided herein. The composition includes an extracellular matrix and at least one exogenous component selected from heparin, fibronectin and laminin. The composition may have a basic pH. Additionally, provided herein is a cell culturing system including a substrate and a coating thereon of the composition to assess potential stimulators and/or inhibitors for their effects on various cell cultures while increasing the signal dynamic range.
Claims22
A composition for coating a surface comprising:
- (a) a growth factor reduced extracellular matrix extracted from Engelbreth-Holm-Swarm mouse sarcoma tumor;
- (b) fibronectin which is exogenous to said growth factor reduced extracellular matrix;
- and
- (c) heparin which is exogenous to said growth factor reduced extracellular matrix;
- wherein endothelial cell tube formation in the composition is minimal in the absence of an exogenous stimulator.
An extracellular matrix composition for coating a surface comprising:
- (a) a growth factor reduced extracellular matrix extracted from Engelbreth-Holm-Swarm mouse sarcoma tumor;
- (b) fibronectin which is exogenous to the growth factor reduced extracellular matrix;
- and
- (c) heparin which is exogenous to the growth factor reduced extracellular matrix;
- the extracellular matrix composition having a basic pH;
- and
- wherein the growth factor reduced extracellular matrix is present at a concentration of about 10 mg/mL of the composition, the fibronectin which is exogenous to the growth factor reduced extracellular matrix is present at a concentration of about 5 μg /mL to about 20 μg/mL of the composition, and the heparin which is exogenous to the growth factor reduced extracellular matrix is present at a concentration of about 200 μg/mL to about 800 μg/mL of the composition.
A cell culturing system comprising:
- a substrate;
- and
- a polymerized coating formed on the substrate from a coating composition comprising:
- (a) a growth factor reduced extracellular matrix extracted from Engelbreth-Holm-Swarm mouse sarcoma tumor;
- (b) fibronectin which is exogenous to said growth factor reduced extracellular matrix;
- and
- (c) heparin which is exogenous to said growth factor reduced extracellular matrix;
- wherein the polymerized coating is formed on the substrate by incubating the coating composition with the substrate at a temperature from about 22° C. to about 37° C. for about 30 minutes to about 4 hours;
- wherein said polymerized coating includes therethroughout said exogenous components;
- and
- wherein endothelial cell tube formation in the polymerized coating is minimal in the absence of an exogenous stimulator.
Dependencies are inferred from the claim text.