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AssigneeSekisui Chemical Co., Ltd.
InventorsMasahiro Furutani, Junichi Hata, Akiko Togi
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This invention provides a recombinant protein expression system using a host and cell-free translation system, and is capable of universally expressing a large amount of any protein as soluble protein, while preventing toxicity in hosts, formation of inclusion bodies, and decompositions with proteases. Such may be achieved by expressing the desired protein as a fusion protein with chaperoning, such as about 60 kDa molecular chaperons, 60 kDa heat shock proteins, or thermosomes, and accommodating the desired protein inside of a stereostructure of a chaperonin. The present invention provides a process for producing a protein, which comprises transcribing and translating a gene containing a gene encoding the linked chaperonin subunits and a gene encoding a desired protein thereby synthesizing a fusion protein having the desired protein linked via a peptide linkage to the linked chaperonin subunits.
A fusion protein consisting of 7 chaperonin subunits and at least one desired protein,
A fusion protein consisting of 2, 4, or 8 chaperonin subunits and at least one desired protein,
A fusion protein consisting of 3 or 9 chaperonin subunits and at least one desired protein,
The fusion protein according to claims or 1, 2, or 3, wherein the chaperonin ring has formed a 2-layer structure associated non-covalently via a ring plane.
The fusion protein according to claims or 1, 2, or 3, wherein the chaperonin rings have assembled into a fibrous structure linked non-covalently via a ring plane or its side.
Dependencies are inferred from the claim text.