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US 7,608,420 B2Granted
Process for the recovery of staurosporine from a fermentation broth
AssigneeLonza Ltd.
InventorsKurt Eyer, Georges Kalbermatten, Jean-Peal Roduit, Alain Wellig
Life of this patent
Estimating the term…
Abstract
Staurosporine of the formula (I) is recovered from a fermentation broth in a process comprising an ultrafiltration and a diafiltration step.
Claims16
A process for the recovery of staurosporine of formula:
- from a fermentation broth comprising the steps of:
- i) adding tetrahydrofuran to the fermentation broth;
- ii) ultrafiltering the dilute fermentation broth obtained in step i);
- iii) optionally diafiltrating the retenate obtained in step ii) with a mixture of a water and tetrahydrofuran, and optionally combining the permeates of the ultrafiltration and the diafiltration;
- iv) optionally adjusting the pH of the permeate obtained in step ii) or step iii) to at least 8.5;
- v) concentrating the permeate;
- vi) adjusting the pH of the concentrate obtained in step v) to at least pH 8.5, unless the pH of said concentrate is already at least 8.5;
- and
- vii) collecting the precipitated staurosporine obtained in step vi).
6 more elements
A process for the recovery of staurosporine of formula:
- from a fermentation broth comprising the steps of:
- i) adding tetrahydrofuran to the fermentation broth;
- ii) ultrafiltering the dilute fermentation broth obtained in step i);
- iii) concentrating the permeate obtained in step ii);
- iv) adjusting the pH of the concentrate obtained in step iii) to at least pH 8.5, unless the pH of said concentrate is already at least 8.5;
- and
- v) collecting the precipitated staurosporine obtained in step iv).
4 more elements
A process for the recovery of staurosporine of formula:
- from a fermentation broth comprising the steps of:
- i) adding tetrahydrofuran to the fermentation broth;
- ii) ultrafiltering the dilute fermentation broth obtained in step i);
- iii) adjusting the pH of the permeate obtained in step ii) to at least 8.5;
- iv) concentrating the permeate from step iii);
- v) adjusting the pH of the concentrate obtained in step iv) to at least pH 8.5, unless the pH of said concentrate is already at least 8.5;
- and
- vi) collecting the precipitated staurosporine obtained in step v).
5 more elements
A process for the recovery of staurosporine of formula:
- from a fermentation broth comprising the steps of:
- i) adding tetrahydrofuran to the fermentation broth;
- ii) ultrafiltering the dilute fermentation broth obtained in step i);
- iii) diafiltrating the retenate obtained in step ii) with a mixture of a water and tetrahydrofuran;
- iv) adjusting the pH of the permeate obtained in step iii) to at least 8.5;
- v) concentrating the permeate from step iv);
- vi) adjusting the pH of the concentrate obtained in step v) to at least pH 8.5, unless the pH of said concentrate is already at least 8.5;
- and
- vii) collecting the precipitated staurosporine obtained in step vi).
6 more elements
A process for the recovery of staurosporine of formula:
- from a fermentation broth comprising the steps of:
- i) adding tetrahydrofuran to the fermentation broth;
- ii) ultrafiltering the dilute fermentation broth obtained in step i);
- iii) diafiltrating the retenate obtained in step ii) with a mixture of a water and tetrahydrofuran;
- iv) combining the permeates of the ultrafiltration and the diafiltration;
- v) adjusting the pH of the permeate obtained in step iv) to at least 8.5;
- vi) concentrating the permeate from step v);
- vii) adjusting the pH of the concentrate obtained in step vi) to at least pH 8.5, unless the pH of said concentrate is already at least 8.5;
- and
- viii) collecting the precipitated staurosporine obtained in step vii).
7 more elements
Dependencies are inferred from the claim text.