Methods of identifying compounds that inhibit Notch cleavage
AssigneeBristol-Myers Squibb Company
InventorsCatherine Burton, Charles F. Albright, Judith A. Wardwell-Swanson
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Abstract
In vitro cell-based methods for identifying compounds that inhibit Notch cleavage and methods for identifying γ-secretase inhibitors that exhibit reduced induction of goblet cell metaplasia are provided. Also provided are methods of identifying compounds that inhibit cleavage of γ-secretase substrates other than Notch and homogeneous compositions or cultures of Notch-expressing cells that undergo mucin-2 or mucin-5AC induction in response to a compound known to inhibit Notch cleavage and methods of their generation.
Claims8
A method for identifying a compound that inhibits cleavage of Notch comprising:
- (a) contacting Notch-expressing HT29 cells with a compound that inhibits γ-secretase;
- and
- (b) detecting mucin-2 induction or mucin-5AC induction in said cells as compared to HT29 cells not contacted with said compound, wherein said mucin-2 induction or mucin-5AC induction is indicative of an inhibitor of Notch cleavage, and wherein the Notch-expressing cells express γ-secretase.
Dependencies are inferred from the claim text.