Back to results
US 7,608,402 B2Granted
DNA methylation specific signal amplification
OwnerWeiwei Li, Jessica Liinventors
Life of this patent
Estimating the term…
Abstract
This invention is related to a method for rapidly detecting gene specific methylation through signal amplification using the epi-barcode after hybrid capture of the methylated DNA sequence.
Claims7
A method for amplifying methylation specific signal generated from methylated DNA sequence in a DNA sample comprising steps of:
- a) binding of a nanobead adaptor consisted of a carrier bead, a bridge antibody and holder oligonucleotides to anti-5-methylcytosine antibody bound to said methylated DNA sequence;
- b) binding of an epi-barcode consisting of polynucleotides therefore containing 5-methylcytosine to nanobead adaptor;
- and c) detection of the presence of the epi-barcode with the anti-5-methylcytosine antibody wherein the amount of the epi-barcode bound to nanobead adaptor determines the amplification intensity of methylation specific signal generated from said methylated DNA sequence in the DNA sample.
Dependencies are inferred from the claim text.