Polynucleotides for use tags and tag complements, manufacture and use thereof
AssigneeLuminex Molecular Diagnostics, Inc.
InventorsPetr Pancoska, Vit Janota, Albert S. Benight, Richard S. Bullock, Peter V. Riccelli, Daniel Kobler, Daniel Fieldhouse
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Abstract
A family of minimally cross-hybridizing nucleotide sequences, methods of use, etc. A specific family of 210 24mers is described.
Claims28
An oligonucleotide tag or tag complement for use in a multiplex assay, wherein the tag or tag complement is selected from the group of oligonucleotides consisting of:
- TGAATTGATGAATGAATGAAGTAT,
- (SEQ ID NO. 21)
- TGATGATTTGAATGAAGATTGATT,
- (SEQ ID NO. 23)
- TGATAAAGTGATAAAGGATTAAAG,
- (SEQ ID NO. 24)
- TGATTTGAGTATTTGAGATTTTGA,
- (SEQ ID NO. 25)
- GTATTTGAGTAAGTAATTGATTGA,
- (SEQ ID NO. 28)
- GATTGTATTGAAGTATTGTAAAAG,
- (SEQ ID NO. 31)
- TGATTTGAGATTAAAGAAAGGATT,
- (SEQ ID NO. 33)
- TGATTGAATTGAGTAAAAAGGATT,
- (SEQ ID NO. 34)
- AAAGTTGAGATTTGAATGATTGAA,
- (SEQ ID NO. 37)
- and
- GTATTGTATTGAAAAGGTAATTGA,
- (SEQ ID NO:
- 39)
- including oligonucleotides complementary thereto.
A nucleic acid tag or tag complement comprising an oligonucleotide selected from the group of oligonucleotides consisting of:
- SEQ ID NO:
- 21, SEQ ID NO:
- 23, SEQ ID NO:
- 24, SEQ ID NO:
- 25, SEQ ID NO:
- 28, SEQ ID NO:
- 31, SEQ ID NO:
- 33, SEQ ID NO:
- 34, SEQ ID NO:
- 37 and SEQ ID NO:
- 39, or a sequence complementary thereto, wherein the oligonucleotide is attached to a solid support.
A combination of oligonucleotide tags or tag complements for use in a multiplexed assay comprising at least one oligonucleotide set forth in SEQ ID NOs.:
- 21, 23-25, 28, 31, 33-34, 37 or 39 and an oligonucleotide selected from the group consisting of SEQ ID NOs.:
- 1-210, wherein the combination comprises at least two different oligonucleotides set forth in SEQ ID NOs.:
- 1-210.
A combination comprising a plurality of oligonucleotide tags or tag complements for use in a multiplexed assay, wherein the plurality of tags or tag complements include SEQ ID NOs:
- 21, 23-25, 28, 31, 33-34, 37 and 39 and sequences complementary thereto.
Dependencies are inferred from the claim text.