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US 7,608,256 B2Granted
Methods to increase transgene expression from bacterial-based delivery systems by co-expressing suppressors of the eukaryotic type I interferon response
AssigneeAeras Global TB Vaccine Foundation
InventorsJerald C. Sadoff, Mohamad F. Jamiluddin, Ravi P. Anantha, John F. Fulkerson, Jr.
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Abstract
Bacterial delivery systems with improved transgene expression are provided. The recombinant bacterial delivery systems deliver transgenes of interest and suppressors of the eukaryotic Type I interferon response to eukaryotic cells. Suppression of the eukaryotic Type I interferon response allows improved expression of the encoded transgene.
Claims10
A genetically engineered bacterium, comprising
- nucleic acid sequences encoding
- one or more passenger genes;
- and
- one or more factors that inhibit a mammalian type I interferon response,
- wherein
- said nucleic acid sequences encoding said one or more passenger genes and said nucleic acid sequences encoding said one or more factors that inhibit a mammalian type I interferon response are operably linked to at least one eukaryotic promoter;
- or
- said nucleic acid sequences encoding said one or more passenger genes are operably linked to a eukaryotic promoter and said nucleic acid sequences encoding said one or more factors that inhibit a mammalian type I interferon response are operably linked to a prokaryotic promoter.
5 more elements
Dependencies are inferred from the claim text.