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US 7,608,180 B2Granted
Amperometric sensor
AssigneeDrew Scientific Holdings, Inc.
InventorsKim King Tong Lau, Jonathan Mark Slater
Life of this patent
Estimating the term…
Abstract
An amperometric sensor suitable for determining the concentration of hydrogen peroxide in a sample, the sensor comprising a ferricyanide compound which, in reduced form, functions as a mediator specific to hydrogen peroxide.
Claims26
A method for determining the concentration of H2O2 in a sample containing H2O2, which method comprises:
- providing a sensor comprising 2 or 3 electrodes, one of the electrodes being coated with a ferricyanide compound which in reduced form functions as a mediator selective for hydrogen peroxide, wherein the ferricyanide compound has a low solubility in an aqueous phase,
- applying a potential of about −400 mV to the sensor, such that the oxidised form of the mediator is reduced to the reduced form,
- contacting the sensor with the sample, such that the H2O2 oxidises the reduced form of the mediator to the oxidised form,
- maintaining the applied potential of about −400 mV such that the oxidised form of the mediator generating a current,
- measuring the generated current.
2 more elements
A method for determining the concentration of an analyte in a sample, which analyte is capable of producing H2O2 when contacted with an analyte, the method comprising:
- contacting the sample with an enzyme such that the analyte in the sample reacts with the enzyme to produce H2O2
- providing a sensor comprising 2 or 3 electrodes, one of the electrodes being coated with a ferricyanide compound which in reduced form functions as a mediator selective for hydrogen peroxide, wherein the ferricyanide compound has a low solubility in an aqueous phase,
- applying a potential of about −400 mV to the sensor, such that the oxidised form of the mediator is reduced to the reduced form,
- contacting the sensor with the sample, such that the H2O2 oxidises the reduced form of the mediator to the oxidised form,
- maintaining the applied potential of about −400 mV such that the oxidised form of the mediator generating a current,
- measuring the generated current.
3 more elements
Dependencies are inferred from the claim text.