Mutant glucose dehydrogenase
AssigneeARKRAY, Inc.
InventorsHideaki Yamaoka, Masashi Tsukada
Life of this patent
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Abstract
A mutant glucose dehydrogenase having an improved substrate specificity to glucose, which is a protein comprising the amino acid sequence depicted in SEQ ID NO:3 or an amino acid sequence having the substitution, deletion, insertion or addition of one or more amino acid residues excluding amino acid 365 in the amino acid sequence depicted in SEQ ID NO:3 and having a glucose dehydrogenase activity, the amino acid residue corresponding to amino acid 365 being substituted by other amino acid residue.
Claims25
An isolated mutant glucose dehydrogenase of SEQ ID NO:
- 3, wherein said mutant glucose dehydrogenase consists of a replacement of the amino acid residue at position 365 with another amino acid residue, and/or consists of a substitution, deletion, insertion or addition of 1 to 10 amino acid residues other than the amino acid residue at position 365 with another or other amino acid residue at the 324th, 326th, 333rd, 334th, 368th, 369th, 376th, 377th, 418th, 419th, 436th, 433rd, 448th, 472nd, 475th, 525th or 529th positions in the amino acid sequence of SEQ ID NO:
- 3, wherein said mutant glucose dehydrogenase has an increased substrate specificity to glucose compared to the glucose dehydrogenase of SEQ ID NO:3.
An isolated mutant glucose dehydrogenase of the glucose dehydrogenase having the amino acid sequence of SEQ ID NO:
- 3, wherein said mutant glucose dehydrogenase consists of:
- (i) substitution of the amino acid residue at position 365 in the amino acid sequence of SEQ ID NO:
- 3,
- (ii) substitution of aspartic acid residue for another amino acid residue at position 472, and
- (iii) substitution of histidine residue for another amino acid residue at position 475,
- wherein said mutant glucose dehydrogenase has increased substrate specificity to glucose, compared to the glucose dehydrogenase comprising the amino acid sequence of SEQ ID NO:
- 3.
An isolated FAD-binding type mutant glucose dehydrogenase comprising the amino acid sequence of SEQ ID NO:
- 7.
Dependencies are inferred from the claim text.