Generation of plants with altered oil content
AssigneeAgrinomics LLC
InventorsJonathan Lightner, Jeremy E. Coate, Stephanie K. Clendennen, Nancy Anne Federspiel, Debra K. Schuster
Life of this patent
Estimating the term…
Abstract
The present invention is directed to plants that display an altered oil content phenotype due to altered expression of an ICL nucleic acid. The invention is further directed to methods of generating plants with an altered oil content phenotype.
Claims13
A method of producing an altered oil content phenotype in a plant, said method comprising:
- a) introducing into progenitor cells of the plant a plant transformation vector comprising a CsVMV promoter and a nucleotide sequence that encodes a sequence that encodes an isocitrate lysase (ICL) polypeptide,
- b) growing the transformed progenitor cells to produce a transgenic plant, wherein said polynucleotide sequence is expressed, and
- c) identifying said transgenic plant with an altered oil content phenotype by measuring the oil content in said transgenic plant and comparing it to that of the oil content measured in a control plant.
A transgenic plant comprising a chimeric DNA construct comprising a CsVMV promoter and a DNA encoding an isocitrate lysase (ICL) polypeptide, wherein the ICL polypeptide has greater than 95% sequence identity to the amino acid sequence presented as SEQ ID NO:2, whereby the transgenic plant has an increased level of ICL relative to a non-transgenic plant and wherein oil from the plant has increased content compared to a plant lacking the chimeric DNA construct.
A method of improving the oil content produced from a plant, said method comprising:
- a) introducing into a plant a chimeric DNA construct comprising a plant specific transcription initiation region, a CsVMV promoter and a DNA encoding an isocitrate lysase (ICL) which, when introduced into cells of said plant increases ICL activity in an amount sufficient to increase the quantity of oil produced by the plant compared to a plant lacking the chimeric DNA construct
- b) identifying said plant with an increased oil content by measuring the oil content in said plant and comparing it to that of the oil content measured in the plant lacking the chimeric DNA construct, thereby providing a method of improving the oil content produced from a plant.
Dependencies are inferred from the claim text.