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AssigneesPioneer Hi-Bred International, Inc., E.I. du Pont de Nemours and Company
InventorsVincent Sewalt, Craig Hastings, Robert Meeley, Sabine Susanne Hantke, Rudolf Jung, John D. Everard, Stephen M. Allen
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Compositions and methods for the alteration of the disulfide status of plant proteins are provided. Novel nucleotide molecules, comprising nucleotide sequences for thioredoxin h and NADPH-thioredoxin reductase, and the proteins encoded thereby are provided. The nucleotide sequences can be used to transform plants alone or in combination with other sequences, such as sequences encoding NADPH generating enzymes, to manipulate the thioredoxin h system and alter the protein disulfide status in plants. Transformed plants, plant cells, plant tissues, seed and grain are provided.
An isolated nucleotide molecule comprising a nucleotide sequence selected from the group consisting of:
An expression cassette comprising a nucleotide molecule, said nucleotide molecule comprising a nucleotide sequence selected from the group consisting of:
A non-human host cell transformed with an expression cassette, said expression cassette comprising a nucleotide molecule, wherein said nucleotide molecule comprises a nucleotide sequence selected from the group consisting of:
A transformed plant comprising in its genome at least one stably incorporated nucleotide construct comprising a promoter that drives expression in a plant cell operably linked to a nucleotide sequence, said nucleotide sequence selected from the group consisting of:
A transformed seed of a plant, said plant comprising in its genome at least one stably incorporated nucleotide construct comprising a promoter that drives expression in a plant cell operably linked a nucleotide sequence, said nucleotide sequence selected from the group consisting of:
A method for altering the disulfide status of storage proteins in a plant or part thereof, said method comprising transforming said plant with a nucleotide construct comprising a promoter that drives expression in a plant cell operably linked to a nucleotide sequence encoding NADPH-thioredoxin reductase, wherein said nucleotide sequence is selected from the group consisting of:
A method for improving the digestibility of grain, said method comprising transforming a plant with a nucleotide construct comprising a promoter that drives expression in a plant cell operably linked to a nucleotide molecule, said nucleotide molecule comprising a nucleotide sequence selected from the group consisting of:
A method for altering grain hardness, said method comprising transforming a plant with a nucleotide construct comprising a promoter that drives expression in a plant cell operably linked to a nucleotide molecule, said nucleotide molecule comprising a nucleotide sequence selected from the group consisting of:
A method for improving grain for processing, said method comprising transforming a plant with a nucleotide construct comprising a promoter that drives expression in a plant cell operably linked to a nucleotide molecule, said nucleotide molecule comprising a nucleotide sequence selected from the group consisting of:
Dependencies are inferred from the claim text.