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US 7,465,810 B2Granted
Reactive 1,3′-crosslinked carbocyanine
AssigneeAnaSpec Incorporated
InventorsZhenjun Diwu, Jianheng Zhang, Yi Tang, Xiang Guobing
Life of this patent
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Abstract
Chemically reactive carbocyanine dyes that are intramolecularly crosslinked between the 1-position and 3′-position, their bioconjugates and their uses are described. 1,3′-crosslinked carbocyanines are superior to those of conjugates of spectrally similar 1,1′-crosslinked or non-crosslinked dyes. The invention includes derivative compounds having one or more benzo nitrogens.
Claims10
A compound of Formula II:
A compound of Formula VI
- and its biological salts,
- wherein C is a non-conjugated chain of 10-50 linear atoms selected from the group consisting of carbon and nitrogen that are further substituted by a hydrogen, an alkyl having 1-20 carbons, a hydroxy,
- X is CR15R16;
- n is 0 to 3;
- R1, R2 and R3 are independently a hydrogen, an alkyl having 1-20 carbons, a cycloalkyl having 3-20 carbons, an aryl, a heteroaryl, an amino, an alkylamino, an arylamino, a thiol, an alkylthiol or a RGM;
- R4-R27 are independently a hydrogen, an alkyl having 1-20 carbons, a hydroxy, an alkoxy having 1-20 carbons, a fluorinated alkyl, a halogen, an alkylthiol, a sulfonyl, a carbonyl, a hydroxy, an amino, an alkylthiol, a thiol, a sulfate, a phosphonate or a RGM;
- RGM is a carboxylic acid, an activated ester of a carboxylic acid, an acyl azide, an acyl nitrile, an imido ester, an isocyanate, an isothiocyanate, or a maleimide.
4 more elements
A compound of Formula VII:
- and its biological salts,
- wherein C is a non-conjugated chain of 10-50 linear atoms selected from the group consisting of carbon and nitrogen that are further substituted by a hydrogen, an alkyl having 1-20 carbons, a hydroxy, and a carbonyl;
- X is CR15R16;
- n is 0 to 3;
- R1, R2 and R3 are independently a hydrogen, an alkyl having 1-20 carbons, a cycloalkyl having 3-20 carbons, an aryl, a heteroaryl, an amino, an alkylamino, an arylamino, a thiol, an alkylthiol or a RGM;
- R4-R23 are independently a hydrogen, an alkyl having 1-20 carbons, a hydroxy, an alkoxy having 1-20 carbons, a fluorinated alkyl, a halogen, an alkylthiol, a sulfonyl, a carbonyl, a hydroxy, an amino, an alkylthiol, a thiol, a sulfate, a phosphonate or a RGM;
- RGM is a carboxylic acid, an activated ester of a carboxylic acid, an acyl azide, an acyl nitrile, an imido ester, an isocyanate, an isothiocyanate, or a maleimide.
4 more elements
Dependencies are inferred from the claim text.