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US 7,465,587 B2Granted
Diagnostic assay device
AssigneeGenzyme Corporation
InventorMichael Reece Imrich
Life of this patent
Estimating the term…
Abstract
This invention relates to assays for an analyte in a liquid sample such as a body fluid. More particularly, the invention relates to a method and apparatus for the detection of a ligand in a body fluid such as urine or blood, which includes an immobilization zone for interfering agents in a sample.
Claims18
An assay device for detection of the presence or absence of an analyte in a sample comprising:
- (a) a sample receiving membrane which conducts flow of a sample, and in flow contact with,
- (b) an analyte detection membrane which conducts flow of the sample comprising an interfering agent immobilization zone at a first situs, a conjugate forming membrane comprising a mobile labeling reagent at a labeling situs located upstream of the interfering agent immobilization zone, and an immobile capture reagent at a capture situs, wherein the labeling reagent is capable of forming a complex with an analyte and an interfering agent, the capture reagent is capable of binding an analyte-labeling reagent complex, and the interfering agent immobilization zone includes a reagent associated therewith that is capable of binding an interfering agent in the sample.
An assay device for detection of the presence or absence of an analyte in a sample comprising:
- (a) a sample receiving membrane which conducts flow of a liquid sample, in flow contact with;
- (b) an analyte detection membrane which conducts flow of the sample comprising an interfering agent immobilization zone at a first situs, a conjugate forming membrane comprising a mobile labeling reagent at a labeling situs, and an immobile capture reagent at a capture situs, wherein the labeling reagent is movable along the membrane and the labeling reagent is capable of forming a complex with an analyte and the interfering agent, the capture reagent is capable of binding an analyte-labeling reagent complex, and the interfering agent immobilization zone includes a reagent associated therewith that is capable of binding an interfering agent-labeling reagent complex thereto;
- and
- (c) an absorbent sink in flow contact with the analyte detection membrane.
1 more element
An assay device for detection of the presence or absence of an analyte in a sample comprising:
- (a) an elongate membrane having, a sample receiving area, a conjugate forming area and an analyte detection area which conductthe flow of a sample therealong, and
- (b) an analyte detection area which conducts flow of the sample comprising an interfering agent immobilization zone at a first situs, a conjugate forming area comprising a mobile labeling reagent at a labeling situs located upstream of the interfering agent immobilization zone, and an immobile capture reagent at a capture situs, wherein the labeling reagent is capable of forming.a complex with an analyte and an interfering agent, the capture reagent is capable of binding an analyte-labeling reagent complex, and the interfering agent immobilization zone includes a reagent associated therewith that is capable of binding an interfering agent in the sample.
Dependencies are inferred from the claim text.