Method and reagent for classifying leukocytes in animal blood
AssigneeSysmex Corporation
InventorsHideaki Matsumoto, Junichi Shiraishi, Hideki Hirayama
Life of this patent
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Abstract
A method for classifying leukocytes in animal blood is described. In the method, a measurement sample is prepared by mixing a canine or feline blood sample with a lysing reagent. Erythrocytes are lysed and leukocytes are shrunk in the measurement sample. The data correlated with the size of leukocytes in the measurement sample are measured. The leukocytes, on the basis of the measured data, are classified into a first group containing lymphocytes, a second group containing neutrophils and monocytes and a third group containing eosinophils.
Claims21
A method for classifying leukocytes in canine or feline blood, comprising the steps of:
- preparing a measurement sample by mixing a canine or feline blood sample with a lysing reagent, the lysing reagent comprising a first alkyl trimethyl ammonium salt having an alkyl group having a carbon number of 10 to 12 and a second alkyl trimethyl ammonium salt having an alkyl group having a carbon number of 16 to 18, wherein erythrocytes are lysed and leukocytes are shrunk in the measurement sample;
- measuring data correlated with the size of leukocytes in the measurement sample;
- and
- classifying the leukocytes, on the basis of the measured data, into a first group consisting essentially of lymphocytes, a second group consisting essentially of neutrophils and monocytes and a third group consisting essentially of eosinophils.
A lysing reagent for classifying leukocytes in a canine or feline blood sample into a first group consisting essentially of lymphocytes, a second group consisting essentially of neutrophils and monocytes and a third group consisting essentially of eosinophils, comprising:
- a quaternary ammonium salt lysing erythrocytes and shrinking leukocytes in the blood sample the quaternary ammonium salt comprising a first alkyl trimethyl ammonium salt having an alkyl group having a carbon number of 10 to 12 and a second alkyl trimethyl ammonium salt having an alkyl group having a carbon number of 16 to 18, and
- an aqueous solvent dissolving the quaternary ammonium salt,
- wherein the concentration of the quaternary ammonium salt is a concentration satisfying the following conditions:
- (1) erythrocytes are sufficiently lysed, and
- (2) leukocytes are shrunk such that the leukocytes can be classified, on the basis of their size, into the first, second and third groups.
A method for classifying leukocytes in animal blood, comprising the step of:
- preparing a measurement sample by mixing a canine or feline blood sample with a lysing reagent, the lysing reagent comprising a first alkyl trimethyl ammonium salt having an alkyl group having a carbon number of 10 to 12 and a second alkyl trimethyl ammonium salt having an alkyl group having a carbon number of 16 to 18, wherein erythrocytes are lysed and leukocytes are shrunk in the measurement sample;
- measuring data correlated with the size of leukocytes in the measurement sample;
- classifying the leukocytes, on the basis of the measured data, into a first group consisting essentially of lymphocytes, a second group consisting essentially of neutrophils and monocytes and a third group consisting essentially of eosinophils;
- and
- wherein the first, second and third groups appear in the order of small to large sizes based on the data correlated with the size of leukocytes.
Dependencies are inferred from the claim text.