Device and method for introducing particle into cell and device and method for collecting particle from cell
AssigneeCanon Kabushiki Kaisha
InventorsSatoru Hatakeyama, Hideaki Okamoto, Norihiko Utsunomiya, Junta Yamamichi
Life of this patent
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Abstract
A device for introducing fine magnetic particles into a cell by applying an electric field to a reaction field where a sample solution containing cells and the fine magnetic particles are introduced, in order to form pores in the surface of the cell, and then applying a magnetic field to the reaction field to introduce the fine magnetic particles into the cell through the pore(s).
Claims3
A method of collecting a magnetic particle out of a cell using a device for introducing the particle into the cell comprising means for forming a pore in the surface of the cell to introduce the particle into the cell, means for introducing the particle into the cell through the formed pore, and means for collecting the particle by taking the particle out of the cell through a pore formed by the means for forming a pore, wherein the means for forming a pore is different from the means for introducing the particle into the cell, the method comprising the steps of:
- forming a pore in the membrane of the cell to collect the particle;
- and
- collecting the particle by taking the particle out of the cell through the pore.
A method of introducing fine particles into a cell and collecting the fine particles, comprising the steps of:
- (1) introducing a sample solution containing cells to a reaction field;
- (2) immobilizing the cells to a carrier;
- (3) applying an electric field to the reaction field to form pores in the cell surface;
- (4) applying a magnetic field to the reaction field to transfer the fine magnetic particles into the cells through pores of the cell surface formed by applying the electric field;
- (5) applying a magnetic field to the reaction field to facilitate the binding of the fine magnetic particles to the desired target substance within a cell, thereby moving the fine magnetic particles within the cell;
- (6) applying an electric field to the reaction field to form pores in the cell surface;
- and
- (7) applying a magnetic field to the reaction field to collect the fine magnetic particles from the cell trough the pores formed in the cell surface by applying the electric field, thereby transferring the fine magnetic particles from the cells.
Dependencies are inferred from the claim text.