Endoglucanases
AssigneeVerenium Corporation
InventorsDavid Lam, Eric J. Mathur
Life of this patent
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Abstract
In one aspect, the invention provides a purified thermostable enzyme derived from the archael bacterium AEPII 1a. In one aspect, the enzyme has a molecular weight of about 60.9 kilodaltons and has a cellulase activity. The enzyme can be produced from native or recombinant host cells, and can be used to aid in the digestion of cellulose. The invention also provides polypeptides having endoglucanase activity having homology to SEQ ID NO:2.
Claims50
An isolated, synthetic or recombinant polypeptide having endoglucanase or cellulase activity comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:46, or encoded by a nucleic acid encoding a polypeptide having endoglucanase or cellulase activity and having at least 95% sequence identity to the polynucleotide sequence of SEQ ID NO:45.
An isolated, synthetic or recombinant polypeptide having endoglucanase or cellulase activity comprising (a) a polypeptide having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:46, (b) a polypeptide encoded by a nucleic acid encoding a polypeptide having endoglucanase or cellulase activity and having at least 95% sequence identity to the polynucleotide sequence of SEQ ID NO:45, or (c) enzymatically active fragments of (a) or (b).
An isolated, synthetic or recombinant polypeptide having endoglucanase or cellulase activity comprising a polypeptide having the amino acid sequence of SEQ ID NO:46, or enzymatically active fragments thereof.
An isolated, synthetic or recombinant polypeptide having endoglucanase or cellulase activity comprising the amino acid sequence of SEQ ID NO:46 and having at least one conservative amino acid substitution, wherein the conservative amino acid substitution comprises:
- a replacement, one for another, among the aliphatic amino acids Ala, Val, Leu and Ile;
- or an interchange of the hydroxyl residues Ser and Thr;
- or an exchange of the acidic residues Asp and Glu;
- or a substitution between the amide residues Asn and Gln;
- or an exchange of the basic residues Lys and Arg;
- or a replacement among the aromatic residues Phe, Tyr,
- wherein the polypeptide has an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:46, or the polypeptide is encoded by a nucleic acid having at least 95% sequence identity to the polynucleotide sequence of SEQ ID NO:45.
An isolated, synthetic or recombinant nucleic acid encoding a polypeptide having endoglucanase or cellulase activity, wherein the nucleic acid hybridizes under stringent conditions to the polynucleotide sequence of SEQ ID NO:45, and the stringent conditions comprise a wash step comprising a wash for 30 minutes at room temperature in a solution comprising 150 mM NaCl, 20 mM Tris hydrochloride, pH 7.8, 1 mM Na2EDTA, 0.5% SDS, followed by 30 minute wash in fresh solution at Tm-10° C.
Dependencies are inferred from the claim text.