Method for calibrating antibiotic disk diffusion testing of microorganisms
AssigneeBioscand AB
InventorGoran Kronvall
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Abstract
A method for calibrating antimicrobic susceptibility testings of microorganisms comprises the steps of creating a histogram with a high response side and a low response side of isolates from a microbial species, which may contain resistant strains against an antimicrobial agent, calculating from the high response side of the histogram at least one statistical parameter, and defining a limit for susceptibility interpretation and comparative analysis of antimicrobic resistance, which is based on the statistical parameter. This limit separates susceptible strains from resistant strains against an antimicrobial agent.
Claims21
A method for calibrating antimicrobic susceptibility testing data of microorganisms, wherein the method comprises the steps of
- creating a histogram based on in vitro susceptibility test data against an antimicrobial agent for isolates from a microbial species, which microbial species isolates may contain unknown resistant strains against said antimicrobial agent wherein the number of isolates of microbial species or percentage of the total number of isolates of microbial species is on a y-axis of said histogram and registered response values against said antimicrobial agent on a x-axis of said histogram;
- determining a position of a response peak of the histogram between a high response side and a low response side corresponding to isolates of microbial species susceptible to said antimicrobial agent;
- calculating from said high response side of said histogram at least one statistical parameter;
- and
- defining a limit for susceptibility interpretation and comparative analysis of antimicrobic resistance, which is based on said at least one statistical parameter;
- said limit separating susceptible strains from resistant strains against said antimicrobial agent.
Dependencies are inferred from the claim text.