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AssigneesToshiyuki Sakai, Chungai Seiyaku Kabushiki Kaisha
InventorToshiyuki Sakai
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A 44-bp region that confers vitamin D3-responsiveness to the human p27Kip1 promoter in the human p27Kip1 transcriptional regulatory mechanism was revealed. This region also conferred vitamin D3-responsiveness to a heterogenous promoter. Moreover, it was found that vitamin D3 enhances the binding between the CCAAT box existing in the region and the NF-Y protein and also the binding between the Sp1 sequence (also existing in the region) and the Sp1 protein, thus inducing human p27Kip1 transcription. Also revealed were methods of screening for pharmaceutical agents that regulate the expression of the p27Kip1 gene using these molecular mechanisms.
An isolated DNA having the activity to give positive vitamin D3 responsiveness to heterologous and homologous promoters, wherein said DNA is selected from the group consisting of:
A vector comprising an isolated DNA consisting of the nucleotide sequence from position 3014 to position 3057 of SEQ ID NO:
A vector comprising an isolated DNA consisting of the nucleotide sequence from position 3014 to position 3057 of SEQ ID NO:
A vector comprising an isolated DNA consisting of three to five tandem copies of the nucleotide sequence from position 3014 to position 3057 of SEQ ID NO:
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