Process for the extraction, purification and enzymatic modification of soy 7S globulin α' subunit for use as hypocholesterolemizing agent
AssigneeIndena S.p.A.
InventorsMarcello Duranti, Paolo Morazzoni
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Abstract
A process for the extraction, purification and enzymatic modification of β-conglycinin α′ subunit, wherein β-conglycinin is selectively extracted from ground, defatted soy, then precipitated by treatment with aqueous ethanol; the enriched fraction is then subjected to Metal Affinity Chromatography (MAC) in denaturant conditions to obtain the α′ subunit, which is treated with chymotrypsin, then subjected to a further MAC to recover the amino-terminal region of this polypeptide (MW 28,000 Da).
Claims11
A process for the selective extraction and purification of soy β-conglycinin α′ subunit, which process comprises the following steps:
- a) extraction of defatted ground soy with a sodium bisulfite aqueous solution at slightly acidic pH to obtain a β-conglycinin-enriched soluble protein fraction;
- b) precipitation of the β-conglycinin fraction from step a) by treatment with ethanol;
- c) purification of the precipitated fraction from step b) by Metal Affinity Chromatography (MAC) under denaturant conditions, to isolate the α′ subunit;
- and
- d) precipitation of the α′ subunit with an organic solvent.
Dependencies are inferred from the claim text.