Acellular antibordetella vaccine
AssigneeInstitut Pasteur
InventorsPascale Gueirard, Nicole Guiso
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Abstract
The invention relates to an immunogenic composition, characterized in that it comprises an adenyl cyclase-hemolysin (AC-Hly) protein, or an immunogenic portion of this AC-Hly, of a strain of Bordetella chosen from B. pertussis, B. parapertussis or B. bronchiseptica, and in that it comprises, in addition, a bacterial extract containing the expression products of the vrg genes of a strain of Bordetella chosen from B. pertussis, B. parapertussis or B. bronchiseptica, or a portion of these expression products which is sufficient to induce an immune response in a host to which the extract might be administered.
Claims10
An immunogenic composition consisting essentially of an immunogenic vrg bacterial urea extract obtained from phase IV strains of at least one of B. pertussis, B. parapertussis, and B. bronchiseptica, wherein said extract comprises at least one expression product of vrg genes from said at least one of B. pertussis, B. parapertussis, and B. bronchiseptica, and wherein said extract is prepared by:
- a) culturing said at least one of B. pertussis, B. parapertussis, and B. bronchiseptica on blood medium to obtain isolated nonhemolytic colonies;
- b) inoculating cells of one or more colonies in liquid medium to give a suspension of cells;
- c) separating said cells from said liquid medium after culture;
- d) suspending the separated cells in a buffer comprising urea for at least an amount of time sufficient to form a bacterial lysate;
- and
- e) separating intact cells and insoluble material from soluble material, wherein said extract comprises said soluble material,
- and wherein the composition is immunogenic when administered to a subject.
Dependencies are inferred from the claim text.