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US 7,425,437 B2Granted
Vaccines against West Nile Virus
AssigneeCrucell Holland B.V.
InventorsAlphonsus G. C. M. UytdeHaag, Govert Johan Schouten, Jaap Goudsmit
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Abstract
The present invention relates to novel vaccines containing (whole-inactivated) West Nile Viruses and/or West Nile viral proteins derived therefrom, produced on human cells, wherein the human cells comprise a sequence encoding at least one early region-1 (E1) gene product of an adenovirus. The cells are preferably cultured in suspension to very high densities and under serum-free conditions. Herein, it is disclosed that use of such cells results in high titers of West Nile Virus produced.
Claims17
A method of producing a West Nile Virus, said method comprising:
- infecting a cell with West Nile Virus, wherein the cell comprises an E1 region of an adenovirus integrated into the cell's chromosomal genome;
- culturing the cell in a suitable medium under conditions that allow for the production of the West Nile Virus;
- and
- producing West Nile Virus.
1 more element
Dependencies are inferred from the claim text.