Method of synthesizing polynucleotides using ionic liquids
AssigneeAgilent Technologies, Inc.
InventorsJoel Myerson, Michel G. M. Perbost, Douglas J. Dellinger, Geraldine F Dellinger
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Abstract
A method of synthesizing polynucleotides is disclosed. The method involves contacting a first nucleotide with a selected reactive group in the presence of an ionic liquid. The selected reactive group may be on a second nucleotide, a polynucleotide, or on a moiety on an insoluble substrate, for example in an oligonucleotide synthesizer.
Claims24
A method of forming an internucleotide bond comprising contacting a free hydroxyl of a growing polynucleotide with a solution comprising a nucleotide monomer and at least 50% by weight of an ionic liquid.
A method of forming an intemucleotide bond between a first nucleoside moiety and a second nucleoside moiety, the method comprising
- (a) dissolving the first nucleoside moiety in a solution comprising at least 50% by weight of an ionic liquid, and
- (b) contacting the second nucleoside moiety with the product of (a) to form the internucleotide bond.
Dependencies are inferred from the claim text.