US 7,410,810 B2Granted

Assay based on doped nanoparticles

AssigneeBayer Technology Services GmbH

InventorsKerstin Bohmann, Werner Hoheisel, Burkhard Köhler, Ingmar Dorn

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Abstract

The invention relates to an assay based on resonance energy transfer (RET), comprising a first molecule grope A, which is marked with at least one energy donor, and at least one second molecule group B which is marked with at least one energy acceptor, the donor comprising a molecule or particle which can be energetically excited by an external radiation source and which is fluorescence enabled and the acceptor comprising a molecule or particle which can be excited by energy transfer via the donor with partial or complete quenching of the donor fluorescence, and the donor and/or acceptor comprise luminescing inorganic dope nanoparticles having an expansion of ≦50 nanometers, emitting electromagnetic radiation with stokes or anti-stokes scattering after energetic excitation.

Claims58

Dependent claims

An assay kit for carrying out an assay method based on a resonance energy transfer (RET), said assay kit comprising:

a) a first molecule group A which is labeled with at least one energy donor (donor), and
b) at least a second molecule group B which is labeled with at least one energy acceptor (acceptor),
wherein the donor comprises a molecule or particle which can be energetically excited by an external radiation source and is capable of fluorescing,
the acceptor comprises a molecule or particle which can be excited by energy transfer by way of the donor, with partial or complete quenching of the donor fluorescence, and
donor and/or acceptor comprise luminescent inorganic doped nanoparticles (lid nanoparticles) which have a breadth of >50 nanometers and which, after an energetic excitation, emit electromagnetic radiation with a Stokes shift or an anti-Stokes shift.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application: 10/494,390Filed 4 Nov 2002
Priority: DE 101 53 8295 Nov 2001
Published: US 2005/0064604 A124 Mar 2005
Granted: 12 Aug 2008
Term ends: Not estimatedThe source records don’t support an estimate.

Classification16 codes

G01N 33/542
With immune complex formed in liquid phase: with steric inhibition or signal modification, e.g. fluorescent quenching
B82Y 30/00
Nanotechnology for materials or surface science
C12Q 1/6818
Characterised by the detection means: involving interaction of two or more labels, e.g. resonant energy transfer
B01J 2219/005
Features relating to the solid phase supports: beads

Family

US 2005/0064604 A1Pre-grant publication · published 24 Mar 2005
US 7,410,810 B2This patent

From the US filing history. Foreign family members aren’t listed.

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