Quantitation of enzyme activity using planar waveguides
AssigneeBayer Aktiengesellschaft
InventorRichard B. Hatch
Life of this patent
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Abstract
Enzyme activity sensors comprising a planar waveguide can be used for sensitive and quantitative measurement of enzyme activity. Such enzyme activity sensors are useful in a variety of settings, including clinical, commercial, and public health settings.
Claims34
An enzyme activity sensor comprising a planar waveguide which comprises a first substrate for a first enzyme and a second substrate for a second enzyme, wherein either:
- (1) the first substrate comprises a first detectable label and the second substrate comprises a second detectable label;
- or
- (2) activity of the first enzyme modifies the first substrate to comprise the first detectable label and activity of the second enzyme modifies the second substrate to comprise the second detectable label;
- and
- wherein the first substrate comprises a first spacer structure located between the surface of the planar waveguide and the first substrate and wherein the second substrate comprises a second spacer structure located between the surface of the planar waveguide and the second substrate, wherein the first spacer structure and the second spacer structure are of different lengths and permit balancing of detectable signals in a multiplexed assay.
An enzyme activity sensor comprising a planar waveguide which comprises a substrate for an enzyme, wherein either:
- the substrate comprises a detectable label;
- or
- activity of the enzyme modifies the substrate to comprise the detectable label;
- and
- wherein the planar waveguide further comprises:
- a macroporous polymer layer which permits detection of enzyme activity in living cells.
An enzyme activity sensor comprising a planar waveguide which comprises a substrate for an enzyme, wherein either:
- the substrate comprises a detectable label;
- or
- activity of the enzyme modifies the substrate to comprise the detectable label;
- and
- wherein the substrate further comprises a spacer structure, wherein the spacer structure is located between the substrate and the enzyme.
Dependencies are inferred from the claim text.