US 7,410,775 B2Granted

Method for making recombinant peptides or proteins using soluble endoproteases

AssigneeBio-Ker S.R.L.

InventorsMarco Vanoni, Paolo Tortora, Giancarlo Tonon, Geoffrey Taylor, Gaetano Orsini

Life of this patent

Estimating the term…

Loading patent…

Abstract

The present invention relates to the expression and secretion in Saccharomyces cerevisiae of readily purifiable soluble variants of the Kex1 endopeptidase of Kluyveromyces lactis and the purification and use thereof for the in vitro processing of recombinant proteins usable in industrial applications. The soluble Kex1 endoproteases described here are free from the transmembrane domain of the native enzyme; the deletion of the transmembrane domain is achieved by removing at least 57 amino acid residues from the C-terminal.

Claims5

Dependent claims

A method for making a recombinant peptide or protein comprising:

(a) producing a fusion protein comprising, at the NH2-terminal end of a peptide or protein of interest, an amino acid sequence containing a dipeptide site hydrolysable by a soluble Kluyveromyces lactis Kex1 endoprotease free from its transmembrane domain;
(b) incubating the fusion protein of step (a) in the presence of a soluble Kluyveromyces lactis Kex1 endoprotease consisting of the amino acid sequence set forth as amino acid residues 103 through 600 of SEQ ID NO 3, or amino acid residues 103 through 611 of SEQ ID NO 3;
and
(c) separating and purifying the peptide or protein of interest.

1 more element

Dependencies are inferred from the claim text.

Facts

Application: 11/804,557Filed 18 May 2007 · division
Priority: IT MI2000A246516 Nov 2000
Published: US 2007/0298457 A127 Dec 2007
Granted: 12 Aug 2008
Term ends: Not estimatedThe source records don’t support an estimate.

Classification4 codes

C12N 9/60
Derived from fungi: from yeast
C12P 21/06
Preparation of peptides or proteins: produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
C12N
Microorganisms or enzymes
C12P
Fermentation or enzyme-using processes to synthesise a desired chemical compound or composition

Family

PCT/EP01/13257Parent of this division · filed 14 Nov 2001
US 2007/0298457 A1Pre-grant publication · published 27 Dec 2007
Application10/181,277Parent of this division
US 7,241,609 B2Parent of this division
US 2003/0186258 A1Published application
US 7,410,775 B2This patent

From the US filing history. Foreign family members aren’t listed.

Timeline

Citations

Open elsewhere

Building an agent or pulling many patents? Use the API or MCP server instead of scraping pages. Get an API key