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US 7,410,774 B1Granted
Compounds modulating sister chromatid separation and method for identifying same
AssigneeBoehringer Ingelheim International GmbH
InventorsFrank Uhlmann, Kim Nasmyth, Jan-Michael Peters, Irene Waizenegger, Sara Buonomo, Rosemary Clyne
Life of this patent
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Abstract
Method for identifying compounds that interfere with or modulate sister chromatid separation in animal or plant cells by modulating a protease with separin-like cysteine endopeptidase activity. Inhibitors of separin activity are useful in cancer therapy, to prevent birth defects and to increase ploidy in plants.
Claims12
A method for identifying a compound that has the activity of inhibiting sister chromatid separation in eukaryotic cells, said method comprising:
- (a) incubating with a test compound a separin in the presence of a separin substrate, wherein said substrate is a peptide or polypeptide comprising an amino acid sequence EXXR (SEQ ID NO:3), wherein X is any amino acid, and the substrate is capable of being cleaved by the separin;
- and
- (b) determining the inhibiting effect of the test compound on the proteolytic activity of the separin,
- wherein a compound determined in (b) to inhibit the proteolytic activity of the separin has the activity of inhibiting sister chromatid separation in eukaryotic cells.
1 more element
Dependencies are inferred from the claim text.