Test methods and devices

A method, comprising:

• (a) providing a sample obtained from a human female, the sample comprising an analyte compound which is a member of the gonadotrophin family;
• (b) providing a first and a second binding agent, wherein the first binding agent is specific to a first form of the analyte compound characteristic of a menopausal state and the second binding agent is specific to a second form of the analyte compound characteristic of a pre-menopausal or fertile state;
• (c) reacting a first portion of the sample with the first binding agent to form a first binding agent/analyte compound complex and subsequently reacting the first binding agent/analyte compound complex with the second binding agent to form a first binding agent/analyte compound/second binding agent complex;
• (d) reacting a second portion of the sample substantially simultaneously with the first binding agent and the second binding agent to form a first binding agent/analyte compound/second binding agent complex;
• (e) determining the amount of first binding agent/analyte compound/second binding agent/complex formed in step (c) and step (d) and displaying the amounts as a ratio;
• and
• (f) comparing the ratio obtained in step (e) to the ratio obtained from a pre-menopausal control, wherein a difference in the two ratios indicates that the human female is in a post-menopausal state.