US 7,410,765 B2 · System for protease mediated protein expression

Abstract

The present invention provides methods and materials that can be used to detect, examine and exploit protease mediated protein expression. An illustrative embodiment of the invention is a polynucleotide encoding a polypeptide having a plurality of operatively linked elements comprising a polypeptide degradation signal that functions as a proteosome substrate; a polypeptide of interest having an observable activity; and a linker disposed between the polypeptide degradation signal and the polypeptide of interest. The linker comprises a protease recognition motif that is capable of being specifically recognized and cleaved by a protease. The activity of the polypeptide of interest is observed when the motif is specifically recognized and cleaved by the protease; and the activity of the polypeptide of interest is decreased or not observed when the motif is not specifically recognized and cleaved by the protease.

Claims9

Dependent claims

A polynucleotide encoding a protease-regulated polypeptide comprising the following operatively linked elements:

(a) a nucleic acid sequence encoding a protein degradation signal;
(b) a nucleic acid sequence encoding a polypeptide of interest;
and
(c) a nucleic acid sequence encoding a linker set forth as SEQ ID NO:1 disposed between (a) and (b), wherein the linker comprises a protease recognition motif that is cleaved by a protease;
wherein, in the presence of the protease, cleavage of the linker protects the polypeptide of interest from proteasomal degradation;
and, in the absence of the protease, activity of the polypeptide of interest is decreased.

3 more elements

Dependencies are inferred from the claim text.

System for protease mediated protein expression

Life of this patent

System for protease mediated protein expression

Life of this patent

Abstract

Claims9