Method for detecting biomolecules
AssigneeSandia Corporation
InventorsQisheng Huo, Jun Liu
Life of this patent
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Abstract
A method for detecting and measuring the concentration of biomolecules in solution, utilizing a conducting electrode in contact with a solution containing target biomolecules, with a film with controllable pore size distribution characteristics applied to at least one surface of the conducting electrode. The film is functionalized with probe molecules that chemically interact with the target biomolecules at the film surface, blocking indicator molecules present in solution from diffusing from the solution to the electrode, thereby changing the electrochemical response of the electrode.
Claims18
A method for detecting biomolecules, comprising:
- contacting a solution containing target biomolecules and indicator molecules with a sensor platform, said sensor platform comprising a nanoporous film deposited on a conducting electrode, said nanoporous film functionalized with probe molecules that chemically interact with said target biomolecules, wherein said nanoporous film is a self-assembled film, said self-assembled film comprising a precursor sol, said precursor sol comprising an aqueous solution prepared from a surfactant, a solvent, and a compound selected from tetra-alkoxysilanes, an alkoxysilane, and a metal alkoxide, and measuring current across said conducting electrode to detect the presence of said target biomolecules.
A method for detecting biomolecules, comprising:
- contacting a solution containing target biomolecules with a sensor platform, said sensor platform comprising a nanoporous film deposited on a conducting electrode, said nanoporous film functionalized with probe molecules that chemically interact with said target biomolecules, and
- measuring the potential difference between said solution and said conducting electrode to detect said target biomolecules.
Dependencies are inferred from the claim text.